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Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis

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Microbiology of the food chain. Polymerase chain reaction (PCR) for the detection of food-borne pathogens. Detection of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis1 Scope This Technical Specification specifies two horizontal methods for detection of the pathogenic bioserotypes of Y. enterocolitica and one for detection of Y. pseudotuberculosis by using real time PCR based methods. The described methods allow for the detection of the two pathogens in enrichments and allow the isolation of colonies. Y. pestis, the causative agent of bubonic and pneumonic plague harbours a variant of the ail gene as well and will

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